Figure 4.

Expression of NMDAR subunits on cortical progenitors. (A) Representative images of coronal cryosections at 17 gw. Double immunolabeling experiments with antibodies against the NR1, NR2A, and NR2B NMDAR subunits (green) and the progenitor subtypes (red) Pax6 (RGCs), Tbr2 (intermediate progenitors) and Nkx2.1 (interneuron progenitors) in the proliferative VZ/SVZ. Cell nuclei stained blue with BB. Boxed areas are presented on higher magnification in the inset. (B) Quantification of the percentage of Pax6⁺, Tbr2⁺, and Nkx2.1⁺ progenitors expressing the 3 NMDAR subunits in the 3 age groups (n = 3 per age group; P < 0.05); The mean values are plotted; the error bars represent ± the SEM.