Elevated microglia/macrophage number and density in response to
severe chronic hypoperfusion. (a) Confocal images from the corpus
callosum of animals immunostained with Iba1 (green), scale bar:
50 µm. (b) There was a significant increase in microglial numbers
following hypoperfusion (***p = 0.0003). (c) There
was a significant increase in % area of Iba1 immunostaining
following hypoperfusion (**p = 0.009). (d) There
was a significant positive correlation between numbers of microglia
and slowing of peak latency (r = 0.59, p = 0.002).
(e) There was a signification positive correlation between % area of
Iba1 immunostaining and peak latency (r = 0.55,
p = 0.006). (f) There was no significant
association between microglial numbers and slowing of axonal
refractory period (r = 0.4, p = 0.06) (g) and no
association between % area of Iba1 immunostaining and peak latency
(r = 0.29, p = 0.17). Data are presented as
mean ± S.E.M. Student’s t test,
*p < 0.05 **p < 0.01,
***p < 0.001; n = 12 per group.