Axon-glial integrity was damaged by severe hypoperfusion but
unaffected by DMF administration. (a) Confocal images from the
corpus callosum of animals from four experimental groups
immunostained with MBP (red), scale bar: 50 µm. (b) Quantification
of MBP immunostaining sections showing no significant changes in
myelin among the groups. (c) Confocal images from the corpus
callosum of animals from four experimental groups immunostained with
MAG (green), scale bar: 50 µm. (d) MAG immunostained sections
showing changes in myelin in hypoperfused animals. Quantification of
MAG mean intensity shows an overall significant effect of surgery
(F(1-31) = 11.7; **p = 0.002)
Post hoc comparison shows a significant reduction with hypoperfusion
in both vehicle (#p < 0.05) and DMF treated
(#p < 0.05) groups. (e) Confocal images from
the corpus callosum from four experimental groups immunostained for
markers of mature oligodendrocytes (CC1) and oligodendrocyte
precursor cells (NG2). Green: CC1; red: NG2, blue: DAPI, scale bar:
100 µm. (f) There is a significant reduction in the number of CC1
cells following hypoperfusion (F(1-33) = 13.0;
**p = 0.001) Post hoc comparison shows a
significant reduction in the number of CC1 immunopositive cells
following hypoperfusion in DMF-treated mice
(#p < 0.05) but not in vehicle-treated mice
(#p > 0.05). There was significant increase
in NG2-positive cells following hypoperfusion surgery
(F(1-33) = 7.6; **p = 0.009). (g)
APP-immunostained sections showing axonal damage in hypoperfused
animals while minimal immunostaining is detected in shams. Green:
APP. Scale bar: 50 µm. (h) Quantification of axonal bulbs shows an
overall significant effect of surgery (F(1-33) = 4.9;
*p = 0.04). Data presented as mean ± SEM,
Two-way ANOVA followed by Bonferroni post hoc test, sham vehicle
n = 7; sham DMF n = 8; hypoperfusion vehicle n = 13; hypoperfusion
DMF n = 9.