Figure 1.

hrd4–1 phenotypes and complementation by NPL4. The indicated yeast strains were incubated as designated by each labeled plate. (YCP) indicates the hrd4–1 strain was transformed with an ARS/CEN yeast genomic library plasmid, isolated for its ability to complement the hrd4–1 mutation and containing a genomic fragment bearing the NPL4 gene. (YIP) indicates a plasmid bearing only the NPL4 and URA3 genes was integrated at the ura3–52 locus in a hrd4–1 strain. “Lovastatin” indicates the addition of 200 μg/ml lovastatin to the indicated plate.