Figure 7.

Degradation of several cytosolic proteins requires the 26S proteasome, but not Hrd4p. (A) Steady-state fluorescence of GFP fusions bearing different N-terminal amino acids was measured by flow cytometry. (B) Degradation of the UFD substrate, ubiquitin^G76V-GFP, was examined by cycloheximide chase followed by flow cytometry. (C) Cycloheximide chase of ubiquitin^G76V-GFP followed by immunoblotting with the use of anti-GFP antibody. (D) Loss of ubiquitin^G76V-GFP immunoreactivity during cycloheximide chase was determined by densitometric analysis of data obtained in (C).