Table 1.
Yeast Strains
| Strain | Genotype |
|---|---|
| RHY244 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2∷URA3 |
| RHY400 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 |
| RHY554 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd4-1 |
| RHY587 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd1Δ∷URA3 |
| RHY877 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 nup116Δ∷URA3 |
| RHY1172 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG |
| RHY1216 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 pRH696a[fur4-430∷URA3] |
| RHY1220 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd4-1 pRH696a[fur4-430∷URA3] |
| RHY1221 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 ubc7Δ∷HIS3 pRH696a[fur4-430∷URA3] |
| RHY1234 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2 hrd1Δ∷TRP1 |
| RHY1246 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2 hrd4-1 |
| RHY1252 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2∷URA3 hrd4-1 |
| RHY1389 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2 hrd2-1 |
| RHY1628 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2∷URA3 hrd2-1 |
| RHY2458 | MATalpha leu2-3,112 ura3-52∷hmg2-GFP∷URA3 |
| RHY2461 | MATalpha ura3-52∷hmg2-GFP∷URA3 cdc48-2 |
| RHY2472 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PKAR2-GFP |
| RHY2473 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PKAR2-GFP hrd1Δ∷TRP1 |
| RHY2474 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PKAR2-GFP hrd4-1 |
| RHY2476 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PTDH3-Deg1-GFP |
| RHY2477 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PTDH3-Deg1-GFP hrd4-1 |
| RHY2478 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ-4 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PTDH3-Deg1-GFP ubc6Δ∷KanMX ubc7Δ∷HIS3 |
| RHY2479 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG2∷URA3∷PTDH3-Deg1-GFP hrd2-1 |
| RHY2485 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2 pRH1377a[HA-prc1-1∷URA3] |
| RHY2486 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2 pRH1377a[HA-prc1-1∷URA3] hrd4-1 |
| RHY2488 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ-4 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2 pRH1377a[HA-prc1-1∷URA3] ubc6Δ∷KanMX ubc7Δ∷HIS3 |
| RHY2494 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2∷URA3 |
| RHY2495 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2∷URA3 hrd4-1 |
| RHY2496 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2∷URA3∷NPL4 hrd4-1 |
| RHY2497 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3 |
| RHY2498 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2∷URA3 hrd4-1 |
| RHY2499 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 leu2Δ met2 trp1∷hisG ura3-52∷PTDH3-1MYC-HMG2 hrd4-1∷URA3∷NPL4 |
| RHY2659 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd4-1 pRH540b[URA3] |
| RHY2660 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd4-1 pRH1443b[OLE1∷URA3] |
| RHY2661 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 pRH540b[URA3] |
| RHY2662 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 pRH1443b[OLE1∷URA3] |
| RHY2669/DTY11A | MATalpha ade2-1 can1-100 leu2-3,112 trp1-1 |
| RHY2670 | MATalpha ade2-1 can1-100 leu2-3,112 trp1-1 ole1Δ∷LEU2 |
| RHY2673 | MATalpha ade2-1 can1-100 leu2-3,112 trp1-1 pRH540b[URA3] |
| RHY2674 | MATalpha ade2-1 can1-100 leu2-3,112 trp1-1 ole1Δ∷LEU2 pRH1443b[OLE1∷URA3] |
| RHY2675 | MATalpha his3Δ200 leu2Δ1 ura3-52∷hmg2-GFP∷URA3 |
| RHY2676 | MATa leu2Δ1 ura3-52∷hmg2-GFP∷URA3 npl4-1 |
| RHY2677 | MATa his3Δ200 ura3-52∷hmg2-GFP∷URA3 npl4-2 |
| RHY2679/BWG-7a | MATa ade1-100 his4-519 leu 2-3,112 ura3-52 ufd1-1 |
| RHY2680 | MATa ade1-100 his4-519 leu 2-3,112 ura3-52 |
| RHY2681 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-6MYC-HMG2 hrd4-1 pRH590a[NPL4∷URA3] |
| RHY2702 | MATa ade1-100 his4-519 leu 2-3,112 ura3-52∷hmg2-GFP∷URA3 ufd1-1 |
| RHY2703 | MATa ade1-100 his4-519 leu 2-3,112 ura3-52∷hmg2-GFP∷URA3 |
| RHY2704 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG pRH1562a[ubiquitin-R-GFP∷URA3] |
| RHY2705 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd4-1 pRH1562a[ubiquitin-R-GFP∷URA3] |
| RHY2706 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd2-1 pRH1562a[ubiquitin-R-GFP∷URA3] |
| RHY2707 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG pRH1563a[ubiquitin-L-GFP∷URA3] |
| RHY2708 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd4-1 pRH1563a[ubiquitin-L-GFP∷URA3] |
| RHY2709 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd2-1 pRH1563a[ubiquitin-L-GFP∷URA3] |
| RHY2710 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG pRH1564a[ubiquitin-P-GFP∷URA3] |
| RHY2711 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd4-1 pRH1564a[ubiquitin-P-GFP∷URA3] |
| RHY2712 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd2-1 pRH1564a[ubiquitin-P-GFP∷URA3] |
| RHY2713 | MATalpha ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG pRH1565a[ubiquitinG76V-GFP∷URA3] |
| RHY2714 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd4-1 pRH1565a[ubiquitinG76V-GFP∷URA3] |
| RHY2715 | MATa ade2-101 lys2-801 his3Δ200 hmg1Δ∷LYS2 hmg2Δ∷HIS3 met2 ura3-52∷PTDH3-1MYC-HMG hrd2-1 pRH1565a[ubiquitinG76V-GFP∷URA3] |
a
ARS/CEN plasmid.
b
2μ plasmid.