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. 2001 Dec;12(12):4129–4138. doi: 10.1091/mbc.12.12.4129

Figure 2.

Figure 2

Newly synthesized Pma1p associates with rafts, mainly, in the Golgi. (A) WT (NY179) and sec18 (H891) cells were radiolabeled for 5 min at 24 or 37°C and chased for 0 or 45 min at 24 or 37°C. After TX100 extraction (1% at 4°C) and density gradient centrifugation, Pma1p was immunoprecipitated from detergent-resistant (R) and soluble (S) fractions and analyzed by SDS-PAGE and phosphorimaging. ER-to-Golgi transport was monitored by following Gas1p processing from precursor (p) to mature (m) form (right panel). (B) WT (NY179) and sec6-4 (NY778) cells were radiolabeled for 30 min at 24 or 37°C and chased for 1 h at 24 or 37°C. Raft association of Pma1p was analyzed as in A.