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Plasma membrane rerouting of Pma1p in vps1 mutant does not restore its raft association. Pma1 and Pma1-7 association with DRMs was examined in VPS1 and vps1 background. Cells were radiolabeled for 10 min at 24 or 37°C and chased for 30 min at 24 or 37°C. ATPase distribution in R and S fractions was analyzed by immunoprecipitation and phosphorimaging.
