Table 1.
Yeast strains used in this study
| Yeast strain | Genotype | Source |
|---|---|---|
| RH690-15D | Mata his4 leu2 ura3 lys2 bar1 | H. Riezman lab |
| RH3804 | Mata lcb1-100 trp1 leu2 ura3 lys2 bar1 | H. Riezman lab |
| MBY208 | Mata lcb1-100 trp1 leu2 ura3 lys2 bar1 pep4∷URA3 | This study |
| NY179 | Mata leu2-3,112 ura3-52 | P. Novick lab |
| NY778 | Mata sec6-4 leu2-3,112 ura3-52 | P. Novick lab |
| H891 | Mata sec18-1 trp1-289 leu2-3,112 ura3-52 his | S. Keranen lab |
| L3852 | Matα his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 | G. Fink lab |
| L4364 | Mata his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 | G. Fink lab |
| ACY7 | Matα his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 pma1-7 | Chang and Fink (1995) |
| ACY12 | Matα his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 pma1-7 pep4∷URA3 | Chang and Fink (1995) |
| ACX28 | Mata his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 pma1-7 | This study |
| ACX58 | Mata his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 pma1-7 vps1Δ∷LEU2 | This study |
| WLY62 | Mata his3Δ200 lys2Δ201 leu2-3,112 ura3-52 ade2 vps1Δ∷LEU2 | This study |
| RSY1396 | Mata lys2Δ201 leu2-3,112 ura3-52 ade2 gap1Δ∷LEU2 | Ljungdahl et al. (1992) |
| Plasmid | Source | |
| p1513 | pep4∷URA3 | W. Zachariae |
| pPL296 | GAP1:HA LYS2 CEN | Ljungdahl et al. (1992) |
| pCKR3A | vps1Δ∷LEU2 | Nothwehr et al. (1995) |
| pAC49 | AST1 URA3 2 μ | Chang and Fink (1995) |
| pAC64 | AST1∷myc3 LEU2 CEN | Chang and Fink (1995) |