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Effects of MDNCR on Cell Apoptosis
(A–C) Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays (A) and Annexin V-FITC and PI binding followed by flow cytometry (B) and counted (C). (D) The mRNA of apoptosis marker genes (Bcl-2, Bax, Caspase-9, and Caspase-3) was detected using real-time qPCR. Scale bars represent 200 μm. Data are shown as means ± SEM for three individuals. *p < 0.05.
