Figure 6.

MDNCR Binding of miR-133a Relieves Its Inhibition of GosB

(A) RNAhybrid and TargetScan predicted miR-133a binding sites at 18 distinct positions in the GosB 3′ UTR. (B) Cells were co-transfected with the miR-133a mimic and pCK-GosB-3′ UTR-W or pcDNA-MDNCR, and Renilla luciferase activity was normalized to the firefly luciferase activity. (C) The mRNA expression of GosB was detected by real-time qPCR. (D and E) The protein expression of GosB was detected by western blotting (D), and protein band density was also analyzed (E). (F) The role of MDNCR as a ceRNA was detected by immunofluorescence (GosB) and observed under a fluorescence microscope. Scale bars represent 200 μm. Values are means ± SEM for three individuals. *p < 0.05.