Figure 6. Global physical interaction between SIRT1 and H3k4ac/H3k9ac epi-marks across all molecular breast tumor subtypes compared to matched normal tissues.

100 to 500 μg of extracted proteins were at first immunoprecipitated using anti-H3k4ac or anti-H3k9ac Abs (IP), or immunoprecipitated without Ab (IP:--) that served as negative control. The immunoprecipitates were then immunoblotted (IB) with anti-SIRT1 Ab. All experiments were performed in triplicate fashion. N: Normal, T: Tumor.