Figure 7. Inverse correlation between SIRT1 and H3k4ac expression levels in 5 intrinsic breast cancer cell lines compared to normal breast cell line.

Proteins were extracted from MCF10A, MCF-7, T-47D, MDA-MB 453, MDA-MB 231 and MDA-MB 468 cell lines. Equal amounts of extracted proteins were immunoblotted using anti-SIRT1 Ab (120 kDa) and anti-H3k4ac Ab (17 kDa). β-actin (42 kDa) served as an internal loading control.