Figure 7. PMN migration into the interstitium and BALF.

Mice with LPS exposure and treatment with KN62 (1 µM) were injected intravenously with Alexa 633-labeled rat anti-mouse GR-1 antibody and allowed to circulate for 5 min to bind intravascular PMN. After 5 min, mice were killed and BALF and lungs were harvested. BALF was determined by flow cytometry and the lungs were digested. These recovered cells were counted and determined by flow cytometry. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 versus vehicle/LPS. (A) Continuously increasing number of PMNs in the interstitium and in the BALF (alveolar space) after LPS-inhalation during the 48 h observation period. (B) Decreased PMN accumulation in KN62-treated mice compared to control after LPS-inhalation. There was no further increase of PMN accumulation in KN62-treated mice after 4 until 24 hours.