Figure 4.

HMGB1 regulated RAGE and autophagic protein expressions in human RCC cell lines.

Notes: (A) The expression and quantification of HMGB1, RAGE, LC3, and Beclin1 in the A498 cell line were analyzed by Western blot analysis. (B) The expression and quantification of HMGB1, RAGE, LC3, and Beclin1 in the ACHN cell line were analyzed by qRT-PCR. Data are presented as mean ± SD from three independent experiments. *P<0.05, **P<0.01 vs Con-siRNA group; ^##P<0.01 vs Con-Vec group; ^{^}P<0.05, ^{^^}P<0.01 vs sRAGE group.

Abbreviations: Con, control; HMGB1, high mobility group box 1 protein; RAGE, receptor for advanced glycation end products; RCC, renal cell carcinoma; qRT-PCR, quantitative reverse-transcription PCR; siRNA, small interfering RNA.