KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
mouse anti-EGFR antibody, clone 225	Millipore	MABF120
anti-mouse IgG-Alexa Fluor488	Jackson Laboratories	715-545-150
anti-NGFR APC-labelled clone ME20.4	Biolegend	345107
Bacterial and Virus Strains
N/A	N/A	N/A
Biological Samples
N/A	N/A	N/A
Chemicals, Peptides, and Recombinant Proteins
N/A	N/A	N/A
Critical Commercial Assays
C1 single cell mRNA seq IFC	Fluidigm	100–5760
NEBNext Poly(A) mRNA Magnetic Isolation Module	NEB	E7490S
NEBNext Ultra RNA Library Prep Kit for Illumina	NEB	E7530S
Deposited Data
Raw Single cell RNA seq data	this paper	https://www.dropbox.com/sh/tjdv3mgxle30qiv/AAAXdnYJRZzMeZYaQg7YIUUaa?dl=0; GSE99330
Raw Bulk RNA seq data	Shaffer et al. 2017	https://www.dropbox.com/s/ir7fp9ragta8jan/A6_bulk_NoDrugfastq.gz?dl=0
RNA FISH data	Shaffer et al. 2017	https://www.dropbox.com/s/om8uq3z3lxnfdtk/fishSubset.txt?dl=0
Experimental Models: Cell Lines
WM989	Meenhard Herlyn	N/A
NIH 3T3	Raj Lab	N/A
JC4	Raj Lab	N/A
Experimental Models: Organisms/Strains
N/A	N/A	N/A
Oligonucleotides
RNA FISH Probe sequences	Biosearch Technologies	Supplementary Table 4
Recombinant DNA
N/A	N/A	N/A
Software and Algorithms
RNA FISH image analysis software	Raj Lab	https://bitbucket.org/arjunrajlaboratory/rajlabimagetools/wiki/Dentist
scRNA-seq analysis code	This paper	https://www.dropbox.com/sh/scmiu1tbrsxupto/AACTL5iWaW-zxRmAbFXnIwnMa?dl=0
Drop-seq_tools-1.0.1	McCaroll Lab	http://mccarrolllab.com/dropseq/
STAR version 2.4.2a		https://github.com/alexdobin/STAR/releases
mRNASeqHT_demultiplex.pl		https://www.fluidigm.com/c1openapp/scripthub/script/2015-08/mrna-seq-ht-1440105180550-2
HTSeq		https://pypi.python.org/pypi/HTSeq
Other
N/A	N/A	N/A