Figure 2.

Private B‐cell clonotypes overlapping the gut and liver are detected in patients with PSC‐IBD. (A) Representative scatter plot indicates the number of B‐cell clonotypes shared in the gut and liver sample from the same patient (paired). Clonotypes restricted to the gut of patient 1 are shown in blue (y axis), clonotypes in the liver of the same patient are shown in orange (x axis). Shared clonotypes (private clonotypes) detected in both the gut and liver (gut–liver clonotypes) are gated and shown in purple (circles). (B) Representative scatter plot shows the absence of clonotypes shared in a gut and liver sample from different patients (unpaired sample; public gut–liver clonotypes). Gut clonotypes exclusive to patient 1 are shown in blue (y axis), and clonotypes restricted to the liver of patient 2 are shown in orange (x axis). Gate highlights the absence of public gut–liver clonotypes. (C) Number of private (paired samples) and public gut–liver (unpaired samples) clonotypes detected in each patient (n = 10). (D) Proportion (%) of private gut–liver clonotypes found within total clonotypes of each patient (gray bars) and average percentage for all PSC‐IBD samples (n = 10, blue bar). (E) Overall proportion (%) of the total gut (gray circles), and liver (blue squares) clonotypes proportion represented by private gut–liver clonotypes. Horizontal lines and bars indicate the mean, and error bars indicate SD. Statistical significance was determined using non‐parametric unpaired t tests; ****P < 0.0001.