Skip to main content
. 2018 Aug 6;16(8):e2001493. doi: 10.1371/journal.pbio.2001493

Fig 4. Activation of canonical Wnt signaling in FSP1+ fibroblasts reduces the number of preadipocytes and impairs adipogenesis.

Fig 4

(A) GSEA data showing negative enrichment of adipogenesis signature in F-BCA SVF cells compared with the Ctrl SVF cells. (B, C) Ctrl and F-BCA SVF cells were subjected to adipogenic induction. Adipogenesis was assayed with Oil Red O staining (panel B). Scale bar: 100 μm. Oil Red O staining was quantitated by isopropanol extraction (n = 3) (panel C). (D) RT-PCR analyses of adipogenic marker expression in Ctrl and F-BCA SVF cells (n = 3). (E) Western blot analyses of PPARγ and c/ebpα in Ctrl and F-BCA SVF cells 6 days after adipogenic induction. (F) RT-PCR analyses of preadipocyte marker expression in Ctrl and F-BCA SVF cells (n = 3). (G, H) FACS analyses of CD34+Sca1+ cells in the SVF of 4-month-old Ctrl and F-BCA mice (n = 5). Data are presented as mean ± SEM. Statistical analyses were performed with two-tailed unpaired student t test. *p < 0.05; **p < 0.01; ***p < 0.001. Underlying data can be found in S1 Data. CD34, cluster of differentiation 34; Ctrl, control; F-BCA, Fsp1-Cre;Ctnnb1exon 3 fl/+; FACS, fluorescence-activated cell sorting; FSP1, fibroblast-specific protein-1; GSEA, gene set enrichment analysis; NES, normalized enrichment score; PPARγ, peroxisome proliferator-activated receptor-γ; RT-PCR, reverse transcription PCR; Sca1, stem cell antigen 1; SVF, stromal vascular fraction.