Fig 2. Construction of HSV-2 mutant viruses with mutated SIAH binding motifs in ICP0.
(A) Depiction of homologous recombination. The native ICP0 sequence in HSV-2 strain MS was exchanged by a sequence encoding ICP0-eGFP fusion protein. (B) Maps of the various ICP0 constructs. The wild-type gene is located in the HSV-2 TRL region. The various ICP0-GFP DNA sequences were accordingly adjusted to reduce their GC content. The NxN1 and NxN2 mutations in the SIAH-1 binding domains are marked in red. Sequences recognized by Southern blot probes are marked in blue.