Fig 6. HSV-2 replication in the cornea of C57Bl/6J mice.
(A) Schematic representation of the murine ocular HSV-2 infection model. Both corneas of anesthetized mice were scarified. Virus was placed on the eyes using 105 pfu/eye. Ocular swabs were taken at the indicated time points post infection (p.i.). Animals were sacrificed at day 3, 5 and 7 post infection, and trigeminal ganglia (TG) and the brain stem were isolated for further analyses. (B) Ocular swabs from HSV-2-ICP0-GFP (ICP0-GFP) or HSV-2-ICP0NxN1/2-GFP (NxN1/2-GFP) infected animals taken at the indicated time points p.i. were analyzed for viral titers. For every experiment a group of n = 10 mice were infected and experiments were repeated four times. Each symbol represents the mean ± SEM of the titers, determined on Vero cell monolayers. The limit of detection is indicated by a dashed line. Statistical differences were calculated using two-way ANOVA with *** p< 0.001.