Skip to main content
. 2018 Jul 25;14(7):e1007511. doi: 10.1371/journal.pgen.1007511

Fig 9. Overexpression of sclB leads to increased production of emericellamides and upregulation of emericellamide cluster genes.

Fig 9

A) Liquid chromatography coupled to mass spectrometry reveals that the sclB OE strain exhibits increased production of emericellamide A (3), C (4) and D (5) compared to wildtype (WT) and sclB comp during vegetative growth. The ΔsclB strain only produces traces of these emericellamides. Depicted is the base peak chromatogram (BPC, upper part) and extracted ion chromatograms (EIC, lower part) of m/z 610.42 [M+H]+ (3) and m/z 596.40 [M+H]+ (4 and 5), respectively. Strains were grown for 48 h in submerged culture conditions and extracellular secondary metabolites were extracted with ethyl acetate. B) qRT-PCR shows that sclB OE upregulates easA, easB, easC and easD gene expression in comparison to wildtype (WT) during vegetative growth (*** P<0.001). None of these genes is expressed in ΔsclB under these culture conditions, with the exception of easA, which is basally expressed.