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. 2018 Aug 6;13(8):e0201920. doi: 10.1371/journal.pone.0201920

Fig 4. Effects of the combined triple treatment on the intracellular proteins in the PANC-1 cells.

Fig 4

The protein expression levels of p-Akt, LC3-II, PARP, cleaved caspase-9, and cleaved caspase-3 were measured using Western blot analysis. β-actin was used as a loading control. (A) The triple treatment decreased the phosphorylation of Akt protein and increased the conversion of LC3-I to LC3-II. The band intensities of LC3-II and LC3-I were quantified to calculate the LC3-II/LC3-I ratio. (B) Apoptotic proteins were activated after the cells were treated with the combined triple treatment. The cleaved PARP was quantified relative to the full-length PARP. These results suggested that both the apoptosis and the autophagy were triggered in the PANC-1 cells by the triple combined treatment. (*** is used for P < 0.001).