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. 2018 Jul 23;145(14):dev164848. doi: 10.1242/dev.164848

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Expression dynamics of the xylem cell marker PjCESA7 during haustorium development. PjCESA7 expression dynamics in (A) root tip and during haustorium formation (B-E) at the indicated time points. Six Z-stack photos (43 μm thickness in total) were stacked using the maximum projection method. Venus fluorescence is in yellow. Bright-field and Venus fluorescent images are merged. Four out of five hairy roots showed a similar expression pattern. (F-H) Expression pattern of PjCESA7::3xVenus-NLS and PjHB15a::3xmCherry-NLS in the same haustorium at the indicated time points. Venus fluorescence is in green. mCherry fluorescence is in magenta. Bright-field, Venus fluorescent and mCherry fluorescent images are merged. The broken white lines mark the edge of the haustorium. Two out of two hairy roots showed a similar expression pattern. The same haustoria are shown in each time-course observation in B-E and F-H. Scale bars: 100 μm. Pj, Phtheirospermum japonicum root; At, Arabidopsis thaliana root.