Skip to main content
. 2018 Jul 23;145(14):dev164848. doi: 10.1242/dev.164848

Fig. 5.

Fig. 5.

Cell lineage analysis of inner cortex during haustorium development. (A) Schematic view of the estradiol-inducible CRE-Lox system for cell lineage tracking. (B) Expression pattern of AtCo2::3xVenus-NLS in Phtheirospermum japonicum root. (C) Estradiol treatment induced the expression of Venus fluorescent protein in inner cortex. (D-I) Expression pattern of Venus fluorescent protein in inner cortex lineage during haustorium formation at the indicated time points. (H,I) Cross-section of the haustorium shown in G. Broken gray line indicates the position of the cross-section. Venus fluorescence is in yellow. mCherry fluorescence is in magenta. PI staining is in red. Venus fluorescent and PI staining images are merged in B. Venus fluorescent and mCherry fluorescent images are merged in C-H. Venus fluorescent, mCherry fluorescent and bright-field images are merged in I. Two out of two hairy roots showed a similar result. The same haustorium is shown in D-I. Scale bars: 50 μm in B, 100 μm in C-I. Pj, P. japonicum root; At, Arabidopsis thaliana root.