Fig. 6.
Cell lineage analysis of epidermis and stele tissue during haustorium development. (A) Transformed root chimerically expressing Venus fluorescent protein in several cell files in epidermis only. (B-D) Epidermal cell lineage during haustorium formation. Square brackets indicate the intrusive region. Venus fluorescence is in yellow. Bright-field and Venus fluorescent images are merged. (E) Transformed root chimerically expressing mCherry fluorescent protein in epidermis (epi), endodermis (end) and cortex (cor). (F,G) Cell lineage of mCherry-expressing cells in haustorium at 96 hpi. (G) Fluorescent image in F is merged with a bright-field image. The broken white lines mark the edge of the haustorium. Broken gray lines mark mCherry-negative tissue. The same hairy roots are shown in A-D and E-G. Scale bars: 100 μm. Pj, Phtheirospermum japonicum root; At, Arabidopsis thaliana root.