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Cif activates ERK MAPK. (A,B) To measure the effect of Cif on cellular signalling, cells were transfected as indicated, followed by Western blotting of cell lysates with the indicated phospho-specific antibodies. The cells were treated with mTOR inhibitor rapamycin (20 nM) for 33 h or 1 μM MLN4924 for 24 h where indicated.
