Fig. 6.

The effect of Cif on B. thailandensis intracellular replication is not due to ERK MAPK activation. (A) Cells were pre-treated with 10 μM MEK inhibitor U0126 for 2 h prior to B. thailandensis infection. Bacterial load 3 h post-infection was quantified. Fold change was presented as mean±s.d. of biological triplicates. (B) To measure the effect of ERK MAPK activation on B. thailandensis intracellular replication, cells were transfected with the constitutive activator of the ERK pathway, MEK-DD, for 2 days, and infected with B. thailandensis. Bacterial load 15 h post-infection was quantified. Fold change was presented as mean±s.e.m. of four independent experiments.