Fig. 2.

cdc14 null males exhibit decreased sperm competition. (A) An example of a sperm competition assay in which a single y w virgin female (white-eyed) is mated to a single male (white-eyed) for 24 h. The male is then removed and the female is mated to a second male with red-eyes for 24 h. The female is then transferred to a fresh vial and allowed to lay eggs. The female is removed, and offspring are allowed to develop and assessed for eye color (red or white). The assay is repeated using a red-eyed male first and then a white-eyed male. (B) A control experiment was performed using white-eyed y w males for both the first and second males. A second control experiment was performed using red-eyed cdc14 null males for both the first and second males. The cdc14 null males are less competitive compared to control males regardless of whether they are the first or second male to mate. Results for a single representative replicates (n≥15 vials per cross) are shown. Additional data can be found in Fig. S5B. Data were analyzed using a Chi-squared test with Bonferroni correction. Six pairwise comparisons were made. Red-eyed control males were compared to the cdc14 null, rescue, or overexpression males; cdc14 null males were compared to rescue or overexpression male; and rescue males were compared to overexpression males. *P<0.009 and ***P<0.0002.