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. 2018 Jun 15;293(31):12120–12136. doi: 10.1074/jbc.RA118.001805

Figure 6.

Figure 6.

KCNE1 and KCNE2 augments hERG-CM expression. hERG expression is demonstrated by a graph of hERG immunoblot data and representative immunoblot from Western blot analysis of lysate (from NDET lysis) is 2 days post-transfection. Results were obtained from three independent transfections (n = 3) transfecting HEK293T cells in 6-well dishes at ∼60% confluency with 282 ng of hERG construct and 718 ng of N-terminal FLAG-tagged KCNE1, N-terminal FLAG-tagged KCNE2, or GFP. The FLAG-tagged constructs were detected with anti-FLAG M2 antibody (catalogue no. F1804, Sigma) used at 1:1000. hERG-CM expression was significantly augmented when co-expressed with KCNE1 or KCNE2 compared with GFP, p = 0.0146 or 0.0018, respectively, as determined by a two-way ANOVA followed by the Sidak's multiple comparison test and indicated with a number sign (#).