Figure 1.

The Ulp2 C terminus contains three conserved regions with distinct and overlapping functions. A, schematic of the conserved regions of Ulp2. B, sequence alignment of two conserved regions in the C terminus of Ulp2 orthologs; excluding its Csm1-binding domain. C, effect of ulp2 mutations on cell growth in the presence of 5-FOA to acutely remove the complementing WT Ulp2 plasmid. YPD loading control is shown along with growth after 3 and 5 days on 5-FOA plates incubated at 30 °C. D, protein abundance of WT and various mutants of Ulp2 following 5-FOA treatment. Coomassie staining is shown to indicate equal loading. E, flow cytometry of asynchronous yeast cells expressing various forms of the Ulp2 protein following 5-FOA treatment.