Figure 3.

Concomitantly increased phosphorylation of ACLY (Ser-455) and acetylation of H3K9 and H3K27 in primary human knee OA chondrocytes. Whole cell lysates isolated from primary human knee chondrocytes from normal (grade 0-I) and OA (grade IV) donors were studied by Western blotting for ACLY expression and phosphorylation (Ser-455), and H3K9 and H3K27 acetylation. β-Actin and histone H3 served as loading controls. Data in A represent 5 normal and 5 OA donors. Age and gender for normal donors were 26 male, 28 male, 34 male, 44 male, 58 male, and age and gender for OA donors were 58 male, 68 female, 71 female, 75 female, 76 male. Semi-quantitative densitometry analyses of Western blots of primary human knee chondrocytes (B) from 28 normal donors (20 male and 8 female, age 42.3 ± 10.6) and 28 OA donors (10 male and 18 female, age 67.8 ± 7.9) were performed. Student's t test was used for statistical data analysis. p values represent comparison of the mean ± S.E. between normal and OA donors.