Figure 5. IKKα inhibits the JAK2/STAT3 pathway.

(A–B) Liver extracts were prepared 4 hr after PHx and immunoblotted with anti-phospho-JAK2 and anti-phospho-STAT3 antibodies. Phosphorylation of JAK2 (pTyr1007/1008) and STAT3 (pTyr705) was normalized to total JAK2 and STAT3 levels, respectively. IKKα^f/f: n = 6, IKKα^Δhep: n = 6. (C) IKKα and JAK2 were coexpressed in HEK293 cells. Cell extracts were immunoprecipitated (IP) and immunoblotted with the indicated antibodies. (D) STAT3 and JAK2 were coexpressed with IKKα in HEK293 cells. Cell extracts were immunoblotted with the indicated antibodies. Data were statistically analyzed with two-tailed Student’s t test, and presented as mean ± SEM. *p<0.05.

Figure 5—figure supplement 1. The effect of PHx on activation of liver IKKα and JAK2/STAT3 pathways.

IKKα^f/f and IKKα^Δhep male mice were subjected to PHx, and livers were harvested on days 0–7. (A) Liver extracts were immunoblotted with the indicated antibodies. (B) Phosphorylation of JAK2 (normalized to total JAK2 levels) and STAT3 (normalized to total STAT3) were analyzed using ANOVA (n = 3 per group). IKKα phosphorylation (Figure 2—figure supplement 1C) was replotted here. (C–D) Primary hepatocytes were transduced with GFP or NIK adenoviral vectors and stimulated with IL6 (10 ng/ml) for 15 min. Cell extracts were immunoblotted with the indicated antibodies. Phosphorylation of STAT3 were normalized to total STAT3 (n = 3 per group). Data were statistically analyzed with two-tailed Student’s t test, and presented as mean ± SEM. *p<0.05.