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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Curr Protoc Cell Biol. 2018 Mar;78(1):20.11.1–20.11.14. doi: 10.1002/cpcb.39

Figure 3.

Figure 3

Plasmid maps. Abbreviations: ApR, bacterial ampicillin resistance gene; BGH pA, HSVTk pA, and SV40 pA, corresponding polyadenylation signals; CMV, EF1a, and SV40, corresponding promoters; F1 ori, single-stranded origin of replication of the bacteriophage F1; MTS, mitochondrial matrix targeting sequence of human ornithine transcarbamylase; myc, myc tag epitope; mUNG1, a gene encoding Y147A mutant of the human UNG1; NeoR, G418 and kanamycin resistance gene; ColE1ori, bacterial origin of replication; qPCR mNuclear, qPCR mMito, qPCR hNuclear, qPCR hMito, fragments encompassing nuclear and mitochondrial targets in mouse and human genomes, respectively.