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STUB1 regulated metformin sensitivity through cyclin A. MDA-MB 231 and Hs578T cells were transfected with negative control, STUB1, and cyclin A siRNA; MDA-MB 231 and Hs578T cells were transfected with empty vector, STUB1, and cyclin A plasmids. (A) MTS cytotoxicity assay was performed. (B) Knockdown efficiency and overexpress efficiency were assessed by qRT-PCR and western blot.
