Skip to main content
. 2016 Oct 23;25(23):5167–5177. doi: 10.1093/hmg/ddw331

Figure 1.

Figure 1.

11β-HSD2 is present in both human and mouse cultured myotubes and is increased in dystrophic skeletal muscle tissues. (A) 11β-HSD2 (44 kDa) was detected by western blots of human primary skeletal muscle (HSMM), C57/BL10 (C57), and mdx (MDX) myotubes differentiated for 5 days using equivalent amounts (35 µg) of cell lysates. Kidney is shown as a positive control tissue. GAPDH (36 kDa) shows equivalent loading of samples between C57 and MDX. (B) Representative western blot of quadriceps muscles from 3 biological replicates shows 11β-HSD2 protein levels from equivalent amounts (50 µg) of protein homogenates from: C57BL/10 wild-type control mice (C57), dystrophin-deficient mdx mice (MDX), dystrophin-deficient; utrophin haplo-insufficient mice (HET), and dystrophin/utrophin-deficient double knockout mice (DKO). Alpha-sarcomeric actin antibody (42 kDa) was used as a loading control.