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. 2016 Oct 23;25(23):5167–5177. doi: 10.1093/hmg/ddw331

Figure 4.

Figure 4.

CYP11B2, but not CYP11B1 protein levels are increased in dystrophic skeletal muscle tissues compared to wild-type. Representative western blots of (A) CYP11B2 protein levels from equivalent amounts of protein homogenates from C57 and HET mouse: quadriceps, tibialis anterior, heart, diaphragm, gastrocnemius, extensor digitorum longus and soleus muscles; (B) CYP11B1 protein levels from equivalent amounts of protein homogenates from C57 and HET mouse: quadriceps, tibialis anterior, heart, and diaphragm muscles from 2 biological replicates. 50 µg of total protein was loaded for all lanes and standardization of loading was verified using an anti-GAPDH antibody (36 kDa). Adrenal glands were included as positive controls.