Figure 7.

Enzymes necessary for local aldosterone synthesis are present in muscle-derived leukocytes. Schematic diagram depicting the aldosterone synthesis pathway and enzyme required for each step. Presence of each enzyme was confirmed by sequencing of RT-PCR products. Yellow star = detected in muscle cultures (myotubes generated from dystrophin-deficient mdx mouse neonatal myoblasts), green star = detected in immune cells (leukocytes isolated from C57BL/10 wild-type mice), and red star = detected in whole muscle tissue (diaphragm muscle tissue from dystrophin/utrophin-deficient double knockout dko mouse).