Figure 1.

Storage of lung grafts in AAT containing Perfadex results in high AAT levels after 4 hours of reperfusion. (A) Experimental set-up for perfusion, cold ischemic storage, transplantation, and reperfusion. Donor lungs were perfused with AAT^wt or albumin in Perfadex and stored in the respective solutions at 4°C for 18 hours (cold ischemia). Thereafter, the left lung was orthotopically transplanted to the C57BL/6J recipient mice. The transplanted animals were kept alive for 4 hours (reperfusion) before Western blot analysis. (B) Western blot of total lung tissue lysates of transplanted left lungs of 4 C57BL/6J recipient mice after 4 hours of reperfusion using an AAT-specific monoclonal antibody. Immunodetection of β-actin served as a loading control (lower panel). The AAT-specific antibody did not cross-react with endogenous mouse proteins (left panel).