Fig. 3.

Functional characterisation of furosemide on the α7 nAChR. A) Representative traces illustrating responses of α7 nAChRs to ACh (100 μM; left), together with ACh responses from the same oocyte after pre- and co-application of furosemide (1 mM; middle). Also shown (right) is a response to ACh (100 μM) following wash. The vertical scale bar corresponds to 200 nA and the horizontal scale bars to 2.5 s. B) Responses with varying concentrations of ACh in the absence (open circles) and presence (closed circles) of a fixed concentration (1 mM) of furosemide. Data are normalised to the maximum ACh response and are means ± SEM of three independent experiments. C) Concentration-response data illustrating potentiation of responses to ACh (50 μM) by varying concentrations of furosemide. Data are normalised to the EC₂₀ concentration of ACh (50 μM) and are means ± SEM of three independent experiments.