Fig. 6.

Functional characterisation of DB04763, DB08122 and pefloxacin on the α7 nAChR. A-C) Representative traces, from oocytes expressing the α7 nAChR, in response to ACh (100 μM; left), together with an ACh response from the same oocyte after pre- and co-application of test compound (1 mM; middle). Also shown are responses to ACh (100 μM) after a 2 min wash (right). Data are shown for DB04763 (A), DB08122 (B) and pefloxacin (C), all at 1 mM. Vertical scale bars correspond to 500 nA and horizontal scale bars correspond to 5 s. D) Concentration-response data illustrating antagonism of responses to ACh (100 μM) by varying concentrations of DB04763 (filled circles), DB08122 (open circles) and pefloxacin (crossed circles). Data are normalised to the response to an EC₅₀ concentration of ACh (100 μM) and are means ± SEM of three independent experiments. E) Responses to varying concentrations of ACh in the presence of a fixed concentration (100 μM) of DB04763 (filled circles), DB08122 (open circles) and pefloxacin (crossed circles). Data are normalised to the response to a maximum concentration of ACh (3 mM) and are means ± SEM of 3–4 independent experiments.