Fig. 7.

Functional characterisation of DB04763, DB08122 and pefloxacin on the 5-HT_3AR and α7/5-HT_3AR chimera. The effects of DB04763 (A), DB08122 (B) and pefloxacin (C), were examined by pre- and co-application (100 μM) with a maximal concentration of agonist (3 mM ACh for α7 and α7/5-HT_3AR chimera; 30 μM 5-HT for 5-HT_3AR) on the α7 nAChR, 5-HT_3AR and α7/5-HT_3AR chimera. All three of the compounds inhibited the 5-HT_3AR and α7/5-HT_3AR chimera to a significantly lower extent than was observed with the α7 nAChR. Data are means ± SEM (n = 4–6). Significant differences are indicated (**P < 0.01, ***P < 0.001).