Figure 3. A study that illustrates co-localization of polarity protein Crb3 and vimentin in the seminiferous epithelium of adult rat testes.
Crb3 (green fluorescence) and vimentin (red fluorescence) were visualized in the seminiferous epithelium of adult rat testes using corresponding specific antibody and/or reagents as described [6, 39]. It was noted that Crb3 co-localized with F-actin, almost superimposable, in the epithelium during the epithelial cycle of spermatogenesis. Cell nuclei were stained by DAPI (4′,6-diamidino-2-phenylindole). Scale bar, 40 μm, which applies to all other micrographs.