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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Hepatology. 2018 May 21;68(2):561–573. doi: 10.1002/hep.29832

Figure 5. Effect of experimental downregulation of Exportin-5 in normal cholangiocytes.

Figure 5

The normal cholangiocyte cell line NHC was stably transfected with scrambled (NHC-SCR) or specific shRNA targeting XPO5 (NHC-XPO5). A, The effect of XPO5 overexpression on the ciliary phenotype was measured by confocal immunofluorescence microscopy where cilia are stained in red with the ciliary marker acetylated-a-tubulin and nuclei in blue with DAPI (*p<0.05, n=25). B, Proliferation rates were assessed by MTS assay (*p<0.001, n=24). C, Protein levels of XPO5 and acetylated-a-tubulin were assessed by western blots (*p<0.05, n=3). D, Total levels of miR-433 and pri-miR-433 were analyzed by qPCR (p<0.001, n=3). E, Nuclear levels of pre-miR-433 and pre-miR-22 were assessed by qPCR on isolated nuclear fractions (p<0.001, n=3).