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. 2018 Mar 14;5:244–256. doi: 10.1016/j.mex.2018.03.002

Fig. 1.

Fig. 1

Schematic representation of the strategy for the genotypic and phenotypic analysis on single zebrafish larvae. (A) Larval tail transection. (B) DNA is extracted from the tail biopsies and genotyping is performed by RFLP assay. (C) Histological study of the anterior part of the zebrafish larvae after paraffin embedding. The red asterisk shows the zebrafish larvae in the paraffin block. (D) Protein analysis by Western blot after total protein or histone extractions from the anterior part of a single zebrafish larvae. (E) Transcript analysis by RT-PCR after RNA extraction from the anterior part of a single zebrafish larvae.