Fig. 2.

Study of caudal spinal cord regeneration. (A) Caudal spinal cord transection on zebrafish embryo at 3 dpf. The dotted line represents the site of transection and the red arrowhead highlights the limit of blood circulation. (B) Picture of the caudal part of a transected zebrafish embryo at 3 dpf. (C) Embryonic caudal region of transected zebrafish embryos at 5 dpf, 2 days post-amputation, showing complete (left) or impaired (right) spinal cord regeneration. Red dotted lines indicate the site of transection. (D) Genotyping performed by RFLP on the caudal part of the embryos after transection at 3 dpf.