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. 2018 Aug 6;8:11765. doi: 10.1038/s41598-018-30040-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup and analysis parameters of the InMAC assay. THP-1 cells are differentiated for 24 hrs followed by o/n infection with d7 promastigotes. Infected THP-1 cells are plated and incubated with the “Leishbox” compounds and controls for 96 hrs. Finally plates are stained with Draq5, read and analysed. Images were taken by Opera at 40x magnification. The infected cells are shown in red while the non-infected ones are shown in green.