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. 2018 Aug 6;8:11728. doi: 10.1038/s41598-018-30020-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CalR-positive cell populations have a diencephalic identity. (A–C) ISH for Foxg1 (A), CalR (B), and Vglut2 (C) in the E14.5 forebrain. Vglut2, but not Foxg1, was expressed in the TE marked by CalR expression. (D,E) ISH for Foxg1 (D) or Vglut2 (E) followed by CalR immunostaining. CalR-expressing cell populations expressed Vglut2, but not Foxg1 (arrowheads). Boxed areas in D and E are magnified in D’ and E’, respectively. TE, thalamic eminence; VZ, ventricular zone. Scale bars: 200 µm in A-C and 500 µm in D,E.