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. 2017 Jun 19;15(6):630–639. doi: 10.1038/cmi.2017.36

Figure 4.

Figure 4

Role of CD4+ T cells in establishing and storing obesity memory. CD4+T cell-deficient H2A−/− mice were subjected to the weight gain-loss-regain cycle. Body weight curve (a) and body weight increment ratio values during the weight regain period (b) were recorded to examine obesity memory in H2A−/− mice. MOH C57BL/6J mice were injected with an anti-CD4 antibody (dMOH) or corresponding isotype IgG (sMOH) to determine the role of CD4+ T cells in obesity memory. CTR C57BL/6J mice were injected with saline. The injection time is indicated with a black arrow. Body weight curve (c) and body weight increment ratio values during the weight regain period (d) were recorded to examine obesity memory. Adoptive transfer of CD4+ T cells was conducted to confirm their role in memorizing obesity. The body weight curves of donor C57BL/6J (e, h) and recipient Rag1−/− mice (f, i) and the body weight increment ratios of recipients during the HFD feeding period (g, j) were recorded after weight loss (e–g) and after 1 month of weight maintenance (h–j), respectively. The cell collection time is indicated by a black arrow. Data are shown as means±s.d. *P<0.05, **P<0.01 and ***P<0.001, unpaired Student’s t-test. §§§ P<0.001, two-way ANOVA followed by the Bonferroni post hoc test. CTR, control group; dMOH, CD4+ T cell-deficient MOH group; HFD, high fat diet; mCD4T, CD4+ T cells carrying obesity memory group; MOH, obesity history group; NC, negative control group; Ns, no significance; PC, positive control group; sMOH, CD4+ T cell-sufficient MOH group.