Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 6;8:11769. doi: 10.1038/s41598-018-30066-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

H₂DCFDA-detected ROS in human, bovine and ascidian spermatozoa exposed to metabolic enhancers. Reactive Oxygen Species (ROS) detected by H₂DCFDA in human (A), bovine (B) and ascidian (C) sperm divided in three groups treated with: (1) five metabolic enhancers (vitamins B6 and B12, 5 methyl THF, zinc and N-acetyl cysteine) (TRT group); (2) n-acetyl-cysteine (NAC group); and (3) medium alone (CNTRL group). Mean (±SE) values of ROS were evaluated in H₂DCFDA-loaded spermatozoa, arranged in TRT, NAC and CNTRL groups and then incubated for 0, 90 and 180 min. ROS production was calculated by evaluating fluorescence intensity (Fo) at the emission peak (~520 nm). Statistically significant differences between groups for each evaluated time point are expressed as different capital (A vs. B; P < 0.01) and small (a vs. b; P < 0.05) letters.