Fig. 3. Overexpression of ciRS-7 promotes metastasis of ESCC cells via miR-7 regulation.

a Migration assays of Eca109 and KYSE150 cells after overexpression of ciRS-7, miR-7, or both. Left panel was representative images and right panel was statistical quantification. b Quantification of cell invasion in Eca109 and KYSE150 cells. c Lung metastasis of Eca109 cells after tail vein injection. Scale bars: 1 cm. d Representative metastatic lesions stained by H&E in the lungs of mice 6 weeks after tail vein injection of indicated cells. Scale bars: 100 μm. e Quantitative analysis of number of lung metastatic colonies in each group. Data in a and b represents the mean ± SD of three repeated experiments. Data in e represents the mean ± SD of eight mice. *P < 0.05